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. 2024 Dec 6;13(23):3431. doi: 10.3390/plants13233431

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The viral accumulation in watermelon with knockdown of the expression of ClBBD via VIGS. (a) The mRNA levels of ClsBBD in three knockdown lines were significantly reduced at 15 days post-VIGS; (b) the mRNA levels of ZYMV and ClBBD in three knockdown lines at 10 days post-inoculation with ZYMV at Stages 5d and 15d, respectively; (c) detection of the viral accumulation at 10 dpi with ZYMV via Western blot; (d) the knockdown plants showed better growth than S_GUS plant at 25 dpi with ZYMV at Stage 5d. Bars represent mean ± SD calculated from three independent biological samples. Asterisks indicate the significant difference to S_GUS control; * and ** represent p < 0.05 and 0.01, respectively.

The viral accumulation in watermelon with knockdown of the expression of ClBBD via VIGS. (a) The mRNA levels of ClsBBD in three knockdown lines were significantly reduced at 15 days post-VIGS; (b) the mRNA levels of ZYMV and ClBBD in three knockdown lines at 10 days post-inoculation with ZYMV at Stages 5d and 15d, respectively; (c) detection of the viral accumulation at 10 dpi with ZYMV via Western blot; (d) the knockdown plants showed better growth than S_GUS plant at 25 dpi with ZYMV at Stage 5d. Bars represent mean ± SD calculated from three independent biological samples. Asterisks indicate the significant difference to S_GUS control; * and ** represent p < 0.05 and 0.01, respectively.