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. 2024 Oct 1;134(24):e183161. doi: 10.1172/JCI183161

Figure 1. Shp1 deletion in neutrophils leads to severe pulmonary hemorrhage and increased inflammation after LPS-induced lung injury.

Figure 1

(A–D) Gross lung and bronchoalveolar lavage (BAL) findings after intratracheal LPS in (A and B) Ptpn6fl/fl and (C and D) Ptpn6fl/fl S100A8(Cre+) mice. Quantitative analysis of BAL indicates (E) alveolar hemorrhage, (F) alveolar neutrophilia, (G) increased vascular permeability, and (H) increased BAL NETs in Ptpn6fl/fl S100A8(Cre+) mice compared to Ptpn6fl/fl. (I) Decreased survival in Ptpn6fl/fl S100A8(Cre+) mice after LPS. (J and K) H&E staining of lung from (J) Ptpn6fl/fl and (K) Ptpn6fl/fl S100A8(Cre+) mice showing increased inflammation and alveolar hemorrhage after LPS with the loss of Shp1 in neutrophils. (L and M) Intravital image after LPS challenge with Evans Blue (plasma stain) and Sytox Green (NET stain) in (L) Ptpn6fl/fl and (M) Ptpn6fl/fl S100A8(Cre+) indicating exacerbated vascular leak and NETs in Ptpn6fl/fl S100A8(Cre+) mice. Scale bars: (A and B) 50 mm; (J and K) 500 μm; (L and M) 50 μm. P values are from unpaired 2-tailed t tests on log10-transformed data (E–H) and log-rank test (I). **P < 0.01, ****P < 0.0001.