KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Staphylococcus aureus RN4220	N/A	N/A
Escherichia coli Rosetta 2 (DE3) - Novagen	Millipore Sigma	71400–3
PhiNM1gamma6-GFP	Baca et al.12	N/A
Phi80alpha-vir	Banh et al.29	N/A
PhiNM4gamma4	Banh et al.29	N/A
PhiJ1	Banh et al.29	N/A
PhiJ2	Banh et al.29	N/A
PhiJ4	Banh et al.29	N/A
Chemicals, peptides, and recombinant proteins
ATP	NEB	P0756S
dATP	NEB	N0440S
ITP	Sigma Aldrich	I0879–50MG
dITP	Thermo Fisher	R1191
Adenosine	Sigma Aldrich	A9251–1G
Inosine	Sigma Aldrich	I4125–1G
AMP	Sigma Aldrich	01930–5G
UTP	NEB	N0450L
CTP	NEB	N0450L
cA2	Biolog	C 088
cA3	Biolog	C 362
cA4	Biolog	C 335
cA6	Biolog	C 332
Deposited data
Cad1 apo	This study	EMDB: 45241 and PDB: 9C67
Cad1 ATP asymmetric	This study	EMDB: 45245 and PDB: 9C6F
Cad1 ATP symmetric	This study	EMDB: 45244 and PDB: 9C6C
Cad1 cA4 ATP	This study	EMDB: 45277 and PDB: 9C77
Cad1 cA6 ATP	This study	EMDB: 45466 and PDB: 9CDB
Cad1 RNA-seq raw reads	This study	Deposited in NCBI Sequence Read Archive with BioProject: PRJNA1152273
apo-Cad1-CARF	This study	PDB: 9C6A
Cad1-CARF + cA6	This study	PDB: 9C68
Cad1-CARF-cA4	This study	PDB: 9C69
Oligonucleotides
See Data S1	N/A	N/A
Recombinant DNA
See Data S1	N/A	N/A
Software and algorithms
CCP4i2 Suite	Winn et al.42	https://www.ccp4.ac.uk
Foldseek	van Kempen et al.18	https://search.foldseek.com/search
CRISPRCasTyper	Russel et al.43	https://crisprcastyper.crispr.dk/#/submit
UCSF Chimera	Pettersen et al.44	https://www.cgl.ucsf.edu/chimera/
cryoSPARC	Punjani et al.45	https://cryosparc.com
HISAT2	Kim et al.46	http://daehwankimlab.github.io/hisat2/
Coot	Emsley and Cowtan47	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
REDItools V2	Flati et al.48	https://github.com/BioinfoUNIBA/REDItools
Alphafold 3	Abramson et al.49	https://alphafoldserver.com
Python 3.9	Python	https://www.python.org/downloads/release/python-390/
Prism 10.1.1	GraphPad	https://www.graphpad.com
Prism 9.4.0	GraphPad	https://www.graphpad.com