Southern-blot analysis of transgene in SGΩ-4 plants. Genomic DNA extracted from wild-type or SGΩ-4 plants was digested with EcoRI, HindIII, or XbaI, respectively, and 10 μg of digested DNA was loaded per lane. An EcoRI-digested PCR fragment of the pumpkin GA 20-oxidase gene, including a 3′-specific region (368 bp), was used as a probe.