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. 2001 Jul;126(3):965–972. doi: 10.1104/pp.126.3.965

Figure 2.

Figure 2

Southern-blot analysis of transgene in SGΩ-4 plants. Genomic DNA extracted from wild-type or SGΩ-4 plants was digested with EcoRI, HindIII, or XbaI, respectively, and 10 μg of digested DNA was loaded per lane. An EcoRI-digested PCR fragment of the pumpkin GA 20-oxidase gene, including a 3′-specific region (368 bp), was used as a probe.