Figure 5.

Bavachin promotes ferroptosis by inactivation of the nuclear factor erythroid 2-related factor 2 (Nrf2)/Heme oxygenase-1 (HO-1) pathway. A: Representative RNA stability and reverse transcription quantitative polymerase chain reaction (RT-qPCR) results of the mRNA levels of Nrf2 and HO-1 in hepatocellular carcinoma (HCC) cells incubated with Bavachin. B: Representative western blot results of the protein levels of Nrf2 and HO-1 in HCC cells incubated with Bavachin. C and D: Quantification of western blot results of Nrf2 and HO-1. Compared to the control group, *P < 0.05.