Figure 7.

Ca²⁺ uptake and Na⁺ sensitivity of yeast cells transformed with wild-type LCT1 or N-terminally truncated LCT1 genes. a, ⁴⁵Ca²⁺ uptake of JKmc cells transformed with EV, wild-type LCT1, or N-terminally truncated LCT1 genes (LCT1-115, LCT1-126, compare with Fig. 6) in liquid uracil-free synthetic medium (SD) medium containing 100 μm CaCl₂. Means of nine experiments ± se are shown. b, Growth of G19 cells transformed with EV, wild-type LCT1, or N-terminally truncated LCT1 (LCT-115 and LCT-126) on plates containing AP medium with various concentrations of NaCl. Note that the truncated LCT1 constructs resemble EV on 20 mm NaCl and LCT1 on higher NaCl. Growth after 8 and 14 d is shown to demonstrate sustainability of phenotypes.