Table 1.
Summary of the Published HPLC Methods for the Determination of Nemonoxacin
| Ref. | Detection | Column | Mobile phase | Matrix | Internal standard | Sample (μL) |
LLOQ (ng/mL) |
|---|---|---|---|---|---|---|---|
| This study | Fluorescence (Ex/Em: 285/465 nm) |
C6-phenyl | MeOH:50 mM KH2PO4 with 0.5% TEA (pH7.5)=45:55 (Plasma); 35:65 (Bile) | Plasma Bile |
Gatifloxacin | 50 10 |
5 5 |
| [7] | UV (292 nm) | C18 | ACN:0.2% TEA (pH2.8 adjust with phosphate acid)=22:78 | Plasma | Moxifloxacin | 500 | 100 |
| [8] | MS/MS (m/z 372.2→354.2) | C18 | ACN:0.1% formic acid=18:82 (Plasma); 15:85 (Urine) | Plasma Urine |
Gatifloxacin | 100 150 |
5 5 |
| [9] | MS/MS (m/z 372.1→354.2) | C18 | MeOH:0.1% formic acid=60:40 | Plasma | Gatifloxacin | 100 | 29.2 |
| [10] | MS/MS (m/z 372→311) | C18 | ACN:5 mm ammonium acetate with 1% formic acid=30:70 | Plasma Urine |
Moxifloxacin | 100 100 |
5 200 |
| [11] | MS/MS (m/z 372.4→354.1) | C18 | ACN:0.1% formic acid=16:84 | Feces | Gatifloxacin | 50 | 120a |
| [12] | MS/MS (m/z 372.5→354.5) | C18 | MeOH:0.3% formic acid=gradient | Lung tissueb | Nemonoxacin-D3 | 100 | 20 |
Note: ang/g; blung tissue, bronchial mucosa and bronchial secretions.
Abbreviations: Ex/Em, excitation/emission; MS/MS, tandem mass; MeOH, methanol; TEA, triethylamine; ACN, acetonitrile; LLOQ, lower limit of quantification.