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. Author manuscript; available in PMC: 2024 Dec 15.
Published in final edited form as: Cancer Lett. 2024 Oct 15;605:217303. doi: 10.1016/j.canlet.2024.217303

Fig. 3. Regulation of eIF3a expression.

Fig. 3.

A-C. Effect of PI3K inhibitors or PTEN expression on eIF3a expression. HT29 cells were treated with Wortmannin (A) or LY294002 (B) for various times, or transiently transfected with PTEN cDNA or vector control (C) followed by Western blot analysis of eIF3a, PTEN and GAPDH control. D-E. Effect of APC expression on eIF3a expression. HT29 and CaCo-2 cells were transiently transfected with the cDNA encoding the wild-type APC or vector control followed by Western blot analysis of eIF3a, APC and GAPDH control (D) or real-time RT-PCR analysis of eIF3a mRNA in CaCo-2 cells (E). F-G. APC status and eIF3a expression in vivo. Total RNAs or lysates were prepared from whole intestinal samples of APCmin/+ and APC+/+ mice and subjected to quantitative RT-PCR (F) and Western blot (G) analysis of eIF3a. The eIF3a mRNA level in APCmin/+ were normalized to that in the wild type control APC+/+ mice and Western blot analysis was performed using samples from 2 individual APCmin/+ and 2 control APC+/+ mice. (***p < 0.001).