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. 2024 Dec 11;17:10929–10942. doi: 10.2147/JIR.S491484

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© 2024 Li et al.

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EHJD drug containing serum alleviated lipid accumulation and liver function injury in oleic acid (OA)-induced HepG2 cells through Nrf2/HO-1 pathway. (A) RT-qPCR was performed to verify the silencing efficiency of Nrf2/HO-1 pathway (n=6). **P<0.01 vs Control. (B) Western blot was used to detect the expression of Nrf2 and HO-1 (n=3). (C) Oil red O staining results of OA-induced HepG2 cells (×200, 100 μm). (D–E). ELISA was executed to measure the levels of blood lipid markers (TC, TG) and liver function markers (ALT, AST) (n=6). **P<0.01 vs OA ^##P<0.01 vs si-NC.