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. 2024 Oct 1;36(12):e13450. doi: 10.1111/jne.13450

TABLE 1.

Statistical analysis table.

Title Distribution Test Results p value Power
Figure 1 Input–Output curves Mauchly's test of sphericity failed (p < .001)

2‐way ANOVA

Time

Group

Interaction

Greenhouse–Geisser

F(9,324) = 45.82

F(3,36) = 0.41

F(27,324) = 0.27

.001

.989

.897

1

0.056

0.106

 NA (Not Applicable)
Figure Title Distribution Shapiro–Wilk Test Results p value Power Post hoc Groups 95% confidence interval
Figure 2A E2 (1 nM) on fEPSP Normality passed, p = .16 Paired sample t‐test (two‐sided) t(7) = 1.77 .12 2‐tailed, 95% = 0.40 NA Baseline versus drug

−0.15

1.30
Figure 2B E2 (100 nM) on fEPSP Normality passed, p = .33 Paired sample t‐test (two‐sided) t(3) = 1.51 .22 2‐tailed, 95% = 0.19 NA Baseline versus drug

−0.35

0.97
Figure 2B LTZ (100 nM) on fEPSP Normality passed, p = .48 Paired sample t‐test (two‐sided) t(10) = 2.89 .02 2‐tailed, 95% = 0.74 NA Baseline versus drug

0.003

0.024
Figure 2A,B E2 (10 nM) on fEPSP Normality passed, p = .47 Paired sample t‐test (two‐sided) t(10) = 3.73 .004

2‐tailed,

95% = 0.9

 NA Baseline versus drug

0.01

0.05
Figure 2B E2 + LTZ on fEPSP Normality passed, p = .30 Paired sample t‐test (two‐sided) t(8) = 1.84 .10

2‐tailed,

95% = 0.37

 NA Baseline versus drug

−0.008

0.075
Figure Title Distribution Test Results p value Power Post hoc
Figure 3 LTZ paired‐pulse ratio Mauchly's test of sphericity failed (p < .018)

2‐way ANOVA

Time

Group

Interaction

Greenhouse–Geisser

F(3,18) = 33.0

F(1,6) = 1.03

F(3,18) = 1.26

.001

.35

.32

.99

.14

.18

 NA
Figure Title Distribution Test Results p value Power Post hoc
Figure 4E fEPSP LTP time course in 10‐min bins Mauchly's Test of Sphericity Failed (p < .000)

2‐way ANOVA

Time

Group

Interaction

Greenhouse–Geisser

F(6,216) = 15.93

F(3,36) = 5.62

F(18,216) = 2.784

.001

.003

.004

1

0.92

0.96

 Pairwise with Sidak adjustment
Figure 4E continued: Pairwise comparisons with Sidak adjustment for multiple comparisons:
Time Group Versus Lower Upper p value

Time 1

(Baseline: −10 to 0′)

 Control LTZ 0.004 0.015 .025
E2 0.002 0.013 .129
E2 + LTZ 0.002 0.014 .083
LTZ Control −0.015 −0.004 .025
E2 −0.007 0.003 .98
E2 + LTZ −0.007 0.004 1
E2 Control −0.013 −0.002 .129
LTZ −0.003 0.007 .98
E2 + LTZ −0.006 0.006 1
E2 + LTZ Control −0.014 −0.002 .083
LTZt −0.004 0.007 1
E2 −0.006 0.006 1

Time 2

(0′ to 10′)

 Control LTZ −0.04 0.305 .548
E2 −0.011 0.334 .333
E2 + LTZ −0.107 0.254 .961
LTZ Control −0.305 0.04 .548
E2 −0.134 0.193 1
E2 + LTZ −0.231 0.114 .98
E2 Control −0.334 0.011 .333
LTZ −0.193 0.134 1
E2 + LTZ −0.26 0.085 .888
E2 + LTZ Control −0.254 0.107 .961
LTZ −0.114 0.231 .98
E2 −0.085 0.26 .888

Time 3

(10′ to 20′)

 Control LTZ 0.033 0.458 .139
E2 −0.083 0.342 .776
E2 + LTZ −0.05 0.396 .545
LTZ Control −0.458 −0.033 .139
E2 −0.318 0.085 .828
E2 + LTZ −0.285 0.14 .983
E2 Control −0.342 0.083 .776
LTZet −0.085 0.318 .828
E2 + LTZ −0.169 0.257 .999
E2 + LTZ Control −0.396 0.05 .545
LTZ −0.14 0.285 .983
E2 −0.257 0.169 .999

Time 4

(20′ to 30′)

 Control LTZ 0.13 0.622 .022
E2 −0.049 0.443 .509
E2 + LTZ 0.086 0.603 .058
Lt Control −0.622 −0.13 .022
E2 −0.413 0.054 .565
E2 + LTZ −0.278 0.215 1
E2 Control −0.443 0.049 .509
LTZ −0.054 0.413 .565
E2 + LTZ −0.098 0.394 .79
E2 + LT Control −0.603 −0.086 .058
LTZ −0.215 0.278 1
E2 −0.394 0.098 .79

Time 5

(30′ to 40′)

 Control LTZ 0.15 0.598 .01
E2 −0.049 0.399 .552
E2 + LTZ 0.131 0.6 .019
LTZ Control −0.598 −0.15 .01
E2 −0.412 0.013 .323
E2 + LT −0.232 0.216 1
E2 Control −0.399 0.049 .552
LTZ −0.013 0.412 .323
E2 + LTZ −0.033 0.415 .433
E2 + Let Control −0.6 −0.131 .019
LTZ −0.216 0.232 1
E2 −0.415 0.033 .433

Time 6

(40′ to 50′)

 Control LTZ 0.226 0.625 <.001
E2 0.047 0.446 .099
E2 + LTZ 0.21 0.63 .001
LTZ Control −0.625 −0.226 <.001
E2 −0.369 0.01 .322
E2 + LTZ −0.205 0.194 1
E2 Control −0.446 −0.047 .099
LTZ −0.01 0.369 .322
E2 + LTZ −0.026 0.373 .409
E2 + LTZ Control −0.63 −0.21 .001
LTZ −0.194 0.205 1
E2 −0.373 0.026 .409

Time 7

(50′ to 60′)

 Control LTZ 0.189 0.633 .004
E2 0.007 0.451 .234
E2 + LTZ 0.174 0.639 .006
LTZ Control −0.633 −0.189 .004
E2 −0.392 0.028 .419
E2 + LTZ −0.226 0.217 1
E2 Control −0.451 −0.007 .234
LTZ −0.028 0.392 .419
E2 + LTZ −0.044 0.399 .504
E2 + LT Control −0.639 −0.174 .006
LT −0.217 0.226 1
E2 −0.399 0.044 .504
Figure Title Distribution Test Results p value Power Post hoc
Figure 4F Fiber volley LTP time course in 10‐min bin Mauchly's test of sphericity failed (p < .000)

2‐way ANOVA

Time

Group

Interaction

Greenhouse–Geisser

F(6,216) = 1.79

F(3,36) = 1.53

F(18,216) = 0.96

.10

.22

.51

0.38

0.37

0.38

 NA
Figure Title Distribution Test Results p value Power Post hoc
Figure 4G

fEPSP change

Bar graph last 10 min

Normality and equality of variance passed

Normality, p = .124

Eq of variance: p = .183

 One way ANOVA

F(3,39) = 6.53

 .001 0.92 Tukey HSD
Figure 4G continued
Post hoc Groups p value 95% interval of confidence
Tukey HSD Group Vs Lower Upper p value
Control Let 10.1414 56.4994 .002
Estradiol −9.6459 36.7121 .407
E2 + LTZ 7.0786 55.6992 .007
LTZ Control −56.4994 −10.1414 .002
Estradiol −41.7768 2.2023 .091
E2 + LTZ −25.1105 21.2475 .996
Estradiol Control −36.7121 9.6459 .407
LTZ −2.2023 41.7768 .091
E2 + LTZ −5.3232 41.0348 .181
E2 + LTZ Control −55.6992 −7.0786 .007
LTZ −21.2475 25.1105 .996
Estradiol −41.0348 5.323 .181
Figure Title Distribution Test Results p value Power Post hoc
Figure 4H Fiber volley change bar graph last 10 min

Normality and equality of variance passed

Normality, p = .74

Eq. of variance, p = .92

 One‐way ANOVA F(3,39) = 0.08 .97 0.049 NA

Abbreviations: E2, 17‐β‐estradiol; fEPSP, field excitatory postsynaptic potential; LTZ, letrozole; LTP, long‐term potentiation; NA, not applicable.