FIGURE 5.

Changes in cell signaling after MRTX1133 (M), tipifarnib (T), or combination treatment (M+T). PANC1 cells were treated with 100 nM MRTX1133 and 500 nM tipifarnib alone or in combination for 48 h. RAS-related cell signaling was determined by Western blotting. (A) Representative bands of the proteins investigated. Activated, phosphorylated proteins are marked with the “p-”prefix. (B) Densitometric evaluation of the activation of the represented proteins. Data were normalized to total protein and expressed relative to the control and graphed with GraphPad Prism 5 software (“C”, ±SEM). Data are derived from three independent experiments. Asterisks mark significant differences (p < 0.05) tested with one-way ANOVA followed by Bonferroni’s Multiple Comparison test comparing treatment groups to control samples.