Figure 3.

Characterization of ChoM proteins produced in transgenic tobacco plants by western-blot analysis. A, Nontargeted ChoM proteins in crude leaf extracts. Mature ChoM was encoded by pMON20923. Full-length ChoM was encoded by pMON20913. B, Chloroplast-targeted ChoM proteins in crude leaf extracts. CTP-mature ChoM was encoded by pMON20931. CTP full-length ChoM was encoded by pMON20929. C, Chloroplast-targeted and nontargeted ChoM proteins in crude leaf extracts. Mature ChoM was encoded by pMON33813. CTP1Δ mature ChoM was encoded by pMON33814. Lanes designated Native ChoM contain purified, secreted bacterial cholesterol oxidase as a standard. Lanes designated Control contain extracts from plants transformed with a vector that lacks the choM gene. Numbers on the left without arrows indicate positions of protein M_r size standards in kilodaltons. Numbers with arrows indicate relative mobilities, in kilodaltons, of specific ChoM protein bands.