Total steroid content of transgenic and control tobacco. All data is represented as average micrograms of steroid per gram fresh weight with calculated ses of the mean. A, Total steroid content for control and groups A, B, C, and D. B, Total sterol and stanol content for control and groups A, B, C, and D. Group A plants express the nontargeted choM gene from pMON33813. Group B, C, and D ChoM+ represent R1 transgenic plant lines, which express the chloroplast-targeted choM gene from pMON33814. Group B, C, and D ChoM represent segregating, non-ChoM-expressing R1 transgenic plant lines derived from R0 plants originally transformed using pMON33814. Control represents non-transformed tobacco of the same developmental age as experimental lines.