Figure 2.

Biological characteristics of circ_0023179 in NSCLC cells. (A) qRT-PCR was used to verify the transfection efficiency of two stably transfected cell lines, A549-sh-circ_0023179 and H1299-sh-circ_0023179; (B) Colony formation assays were used to assess the effect of circ_0023179 knockdown on the proliferation ability of NSCLC cells; (C) EdU assays were used to evaluate the proliferation ability of two stably transfected cell lines, A549-sh-circ_0023179 and H1299-sh-circ_0023179; (D and E) Western blot assays were used to detect EMT-related proteins. *P < 0.05, **P < 0.01, ***P < 0.001. NSCLC: Non-small-cell lung cancer; qRT-PCR: Quantitative real-time polymerase chain reaction; EMT: Epithelial–mesenchymal transition.