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. 2025 Jan 1;25(1):155–164. doi: 10.17305/bb.2024.10944

Figure 3.

Figure 3.

circ_0023179 targeted miR-615-5p. (A) The circInteractome and circbank biology websites were used to predict the target miRNAs for circ_0023179; (B and C) A549 and H1299 cells were examined using a circ_0023179 probe in RIP assays; (D) The binding site of circ_0023179 for miR-615-5p was predicted; (E) qRT-PCR was used to detect the expression of miR-615-5p in NSCLC tissues and paraneoplastic tissues; (F) qRT-PCR was used to detect the expression of miR-615-5p in the HBE, A549, and H1299 cells; (G and H) Dual-luciferase reporter assays were used to verify the binding of circ_0023179 and miR-615-5p; (I and J) RNA pull-down assays were used for the biotin-labeled miR-615-5p in A549 and H1299 cells. *P < 0.05, **P < 0.01, ***P < 0.001. miRNA: MicroRNA; RIP: RNA immunoprecipitation; qRT-PCR: Quantitative real-time polymerase chain reaction; NSCLC: Non-small-cell lung cancer.