Skip to main content
. 2025 Jan 1;25(1):155–164. doi: 10.17305/bb.2024.10944

Figure 5.

Figure 5.

CDH3 was a downstream gene of miR-615-5p. (A) Four bioinformatic sites (i.e., targetscan, LUAD, DINAN TOOLS, and LUSC) were used to predict the potential target mRNA of miR-615-5p; (B) The binding sites of miR-615-5p to CDH3; (C) qRT-PCR was used to detect CDH3 expression in the paraneoplastic and NSCLC tissues; (D) Western blot was used to detect the expression of CDH3 protein in the paraneoplastic and NSCLC tissues; (E) qRT-PCR was used to detect the expression of CDH3 in HBE, A549, and H1299 cells; (F) Western blot was used to detect CDH3 protein expression in HBE, A549, and H1299 cells; (G and H) Dual-luciferase reporter assays were used to verify the binding of CDH3 mRNA and miR-615-5p; (I and J) Western blot assay was used to examine the effect of miR-615-5p on CDH3 protein expression in A549 and H1299 cells. *P < 0.05, **P < 0.01, ***P < 0.001. CDH3: Cadherin 3; mRNA: Messenger RNA; qRT-PCR: qRT-PCR: Quantitative real-time polymerase chain reaction; HBE: Human bronchial epithelial.