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. 2001 Jul;126(3):1186–1195. doi: 10.1104/pp.126.3.1186

Figure 2.

Figure 2

Cloning of chicory 1-FEH IIa and IIb. A, Schematic representation of the 1-FEH IIa and IIb cDNAs from chicory. Both cDNAs contain a single open reading frame (Start-Stop) of 1,743 bp. The first part of this open reading frame is a putative signal sequence (black) of 104 bp. 3′- and 5′-untranslated parts are also present (line). Primers used during RT-PCR, PCR, 5′-, and 3′-RACE RT-PCR are indicated with arrows (for details, see “Materials and Methods”). Probes were prepared by using the primers P14-P15 (1-FEH IIa) or P14-P16 (1-FEH IIb). B, Alignment of the deduced amino acid sequences of chicory root 1-FEH IIa, 1-FEH IIb, and 1-FEH I. The posttranslationally removed signal sequences are indicated (underlined and bold). Potential glycosylation sites are double underlined. For 1-FEH IIa and IIb, tryptic fragments found after quadrupole-time of flight (Q-TOF) mass spectrometry/mass spectroscopy (MS/MS) analysis are indicated (bold). Consensus line: asterisks indicate identical residues, colons indicate conserved subsitutions, and periods indicate semi-conserved substitutions.