Table 2.
Cosinor-based rhythmometry analysis of circadian genes.
| Mesor (relative expression) |
Amplitude (relative expression) |
Acrophase (hours) |
Percentage of rhythmic subjects |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Controls | Active CS | CS in remission | Controls | Active CS | CS in remission | Controls | CS in remission | Controls | Active CS | CS in remission | ||
| ARNTL | 1.35 (0.81–1.50) | 1.71 (1.30–3.04)∗∗ | 0.99 (0.60–0.99)°° | 0.55 (0.28–0.75) | 0.33 (0.22–0.63) | 0.34 (0.21–0.49) | 01:03 (22:27–04:47) | 00:22 (20:54–01:03) | 36% | 41% | 38% | |
| CLOCK | 1.46 (1.19–1.69) | 1.37 (0.89–1.68) | 0.95 (0.63–1.12)## | 0.54 (0.36–0.65) | 0.21 (0.15–0.30) | 0.45 (0.32–0.55) | 01:00 (20:49–02:46) | 00:55 (19:40–01:54) | 46% | 33% | 12% | |
| PER1 | 0.34 (0.24–0.49) | 0.16 (0.11–0.70) | 0.66 (0.51–0.77)## | 0.22 (0.18–0.29) | 0.11 (0.05–0.20)∗ | 0.34 (0.22–0.50)° | 03:18 (21:35–05:01) | 19:55 (19:06–21:50) | 73% | 30% | 88% | |
| PER2 | 0.50 (0.32–0.66) | 0.69 (0.47–0.99) | 0.67 (0.57–0.77) | 0.32 (0.19–0.46) | 0.15 (0.09–0.21)∗∗ | 0.30 (0.25–0.34)°° | 02:39 (20:51–04:10) | 22:21 (21:08–00:17) | 55% | 30% | 75% | |
| PER3 | 0.49 (0.28–0.70) | 0.21 (0.12–0.47)∗ | 0.87 (0.69–0.16)°°;# | 0.28 (0.21–0.42) | 0.17 (0.06–0.27)∗ | 0.38 (0.35–0.47)°° | 22:05 (19:49–00:20) | 21:27 (20:15–23:22) | 82% | 41% | 75% | |
| PRF1 | 1.15 (0.91–1.46) | 1.58 (1.08–1.58)∗ | 1.29 (1.07–1.58) | 0.42 (0.25–2.45) | 0.29 (0.13–0.42) | 0.44 (0.27–0.58) | 21:47 (19:40–00:02) | 23:06 (21:24–01:33) | 73% | 26% | 88% | |
| TIMELESS | 0.89 (0.75–1.23) | 1.13 (0.85–1.55) | 1.25 (0.93–1.45) | 0.42 (0.2–0.67) | 0.18 (0.10–0.32)∗∗ | 0.44 (0.40–0.63)°° | 22:57 (20:59–01:21) | 22:05 (19:25–01:10) | 77% | 30% | 63% | |
Results of cosinor-based rhythmometry analysis on the time-course relative expression of circadian genes and percentage of subjects with circadian rhythmicity. Results are expressed as median (25th—75th percentiles). ∗: active CS vs. Controls; °: CS in remission vs. active CS; #: CS in remission vs. controls. ∗,°,#p < 0.05; ∗∗,°°,##p < 0.010; ∗∗∗,°°°,###p < 0.001 (Mann–Whitney test).