Table 4.
Cosinor-based rhythmometry analysis of peripheral blood mononuclear cells.
| Mesor (%) |
Amplitude (%) |
Acrophase (hours) |
||||||
|---|---|---|---|---|---|---|---|---|
| Controls | Active CS | CS in remission | Controls | Active CS | CS in remission | Controls | CS in remission | |
| Total Monocytes | 15.7 (13.8–17.2) | 26.3 (22.4–29)∗∗∗ | 16.8 (14.7–18.4)°° | 3.4 (2.5–1.4) | 2.2 (1.4–3.8) | 4.2 (3.3–7.2)° | 23:53 (22:04–02:07) | 23:01 (21:37–01:23) |
| Classical Monocytes | 89.2 (87.2–90.8) | 81.1 (76.7–83.5)∗∗∗ | 84.2 (81.2–86.6)## | 2.8 (2.1–3.7) | 4.4 (2.3–6.9) | 3.1 (1.8–4.8) | 23:30 (21:11–02:14) | 20:37 (19:11–23:20) |
| Intermediate Monocytes | 3.8 (3.1–4.9) | 5.1 (4.1–7.0)∗∗ | 3.8 (3.3–4.2)° | 1.0 (0.7–1.4) | 1.5 (0.9–2.4) | 0.9 (0.8–2.4) | 23:07 (20:20–01:43) | 02:43 (19:31–04:07) |
| Non-classical Monocytes | 5.0 (3.1–6.2) | 12.6 (9.1–18.2)∗∗∗ | 9,2 (7.3–11.9)## | 1.4 (0.9–2.4) | 3.5 (1.6–5.9)∗∗ | 2.9 (2.3–4.1) | 00:06 (22:34–02:25) | 21:51 (20:08–00:26) |
| Total T Lymphocytes | 57.8 (53.7–61.1) | 35.4 (31.9–44.4)∗∗∗ | 49.6 (42.8–50.7)°;## | 4.8 (2.8–6.5) | 4.4 (2.4–7.9) | 8.1 (6.4–11.1) | 23:48 (20:27–02:36) | 03:29 (01:07–05:01) |
| CD4+ T Lymphocytes | 58.8 (56.0–64.3) | 29.6 (27.9–27.9)∗∗∗ | 53.4 (44–56.8)°°;# | 5.8 (4.4–10.5) | 4.1 (2.6–7.4) | 6.1 (3.7–7.7) | 00:22 (22:43–02:01) | 01:31 (22:38–03:58) |
| CD8+ T Lymphocytes | 36.2 (32.4–40.0) | 67.4 (60.2–69.2)∗∗∗ | 42.2 (39.3–53.2)°°;# | 5.8 (4.6–9.0) | 4.4 (3.0–6.3) | 3.1 (2.8–8.3) | 22:29 (20:02–02:36) | 00:54 (21:40–02:28) |
| Total CD56+ NK cells | 13.0 (11.2–15.4) | 9.3 (6.6–10.7)∗∗∗ | 11.1 (8.7–16.3) | 2.0 (1.3–3.4) | 2.0 (1.3–4.2) | 3.3 (1.5–3.8) | 21:59 (20:24–23:59) | 02:32 (22:37–02:57) |
| CD56bright NK cells | 8.3 (6.6–11.1) | 3.8 (2.6–5.5)∗∗∗ | 2.3 (1.5–3.1)### | 2.4 (1.3–4.1) | 1.7 (1.0–2.4) | 0.7 (0.6–1.1) | 23:08 (20:44–00:46) | 23:04 (23:02–01:12) |
| CD56dim CD16+ NK cells | 87.9 (85.8–91.1) | 54.4 (52.2–62.0)∗∗∗ | 71.3 (56.5–84.6)°°;### | 5.5 (3.1–8.8) | 9.5 (6.1–14.9)∗ | 11.0 (8.1–18.8) | 23:48 (20:43–01:03) | 21:57 (21:02–00:00) |
Results of cosinor-based rhythmometry analysis on time-course percentages of immune cells. Results are expressed as median (25th—75th percentiles). ∗: active CS vs. Controls; °: CS in remission vs. active CS; #: CS in remission vs. controls.
∗,°,#p < 0.05; ∗∗,°°,##p < 0.010; ∗∗∗,°°°,###p < 0.001 (Mann–Whitney test).