Figure 3.

Free Mn and Rho do not affect each other’s actions.A, the YFP fluorescence (MFI) values in construct three are plotted. 8 mM Mn or 100 μg/ml BCM were used, as indicated. The values are mean ± s.d. values from six independent experiments. ∗∗∗p < 0.001, unpaired t test. B, the relative β-galactosidase activities in construct four containing trp t’ terminator are plotted. 8 mM Mn or 100 μg/ml BCM were used, as indicated. The calculated Miller unit for constructing four without any treatment was 4428 ± 902. The values are mean ± s.d. from three independent experiments. ∗∗∗p < 0.001, unpaired t test. C, the cellular Mn level in the presence or absence of 100 μg/ml BCM are plotted. The calculated mean ± s.d. values from three independent experiments were plotted. ∗∗∗p < 0.001, unpaired t test.