Figure 7.

MntR-mediated upregulation of mntP does not impact the Rho-dependent termination.A, the expression levels of mntP observed in the presence or absence of Mn (8 mM), BCM (100 μg/ml) or both. The fold change values (mean ± s.d.) from three independent experiments were plotted. ∗∗∗p < 0.001, unpaired t test. B, the Western blot experiments show the effect of Mn (8 mM), BCM (100 μg/ml) or both on MntP-FLAG expression. The anti-FLAG band intensities were calculated by Image J software. The band intensities were normalized and plotted. The anti-FLAG band remained below detection level in the untreated sample. The fold change values (mean ± s.d.) are from three independent blots.