Table 1.
The list of strains and plasmids used in this work
| Strains and plasmids | Genotype/Features | References |
|---|---|---|
| Strains | ||
| BW25113 | Escherichia coli; rrnB3ΔlacZ4787hsdR514Δ(araBAD) 567 Δ(rhaBAD)568 rph-1 | (58) |
| ΔmntP | BW25113, ΔmntP::kanR | (58) |
| ΔmntR | BW25113, ΔmntR::kanR | (58) |
| ΔmntH | BW25113, ΔmntH::kanR | (58) |
| ΔRS::kanR | BW25113, ΔRS::kanR; (mntP riboswitch::kanR) | This study |
| ΔRS | BW25113, ΔRS::scar; (kanR was removed) | This study |
| ΔmntR ΔRS | BW25113, ΔmntR::kanR, ΔRS::scar | This study |
| ΔmntR ΔmntH ΔRS | BW25113, ΔmntR::kanR, ΔmntH::kanR, ΔRS::scar | This study |
| BL21 (DE3) | F– ompT hsdSB (rB–, mB–) gal dcm (DE3) | Lab collection |
| C43 (DE3) | A BL21 (DE3) derivative | (59) |
| Lemo (DE3) | BL21 (DE3) containing the Lemo System | (60) |
| Plasmids | Features | References |
| pET28a (+) | kanR; T7-promoter; IPTG inducible | Novagen |
| pET-mntP | mntP without a stop codon cloned in NcoI (blunted) and HindIII sites of pET28a (+) vector | This study |
| pAH125 | kanR; LacZ reporter | (57) |
| pVenus | kanR; YFP reporter | (57) |
| pINT | ampR; λ-integrase | (57) |
| pKD4 | ampR; kanR cassette is flanked by FLP recombinase | (43) |
| pT7A1-trp t’ | T7A1-trp t’ cassette cloned in pGEM-T (Promega) | This study |
| pBAD/Myc-His A | ampR; cloning vector | Invitrogen |
| pBAD-mntP | mntP cloned in NcoI (blunted) and HindIII sites of pBAD/Myc-His A vector | This study |
kanR, kanamycin resistance; ampR, ampicillin resistance.