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. 2024 Nov 9;3(4):100148. doi: 10.1016/j.imj.2024.100148

Table 1.

Detailed information on the HTL, CTL, and B-cell epitopes selected to construct the ZL12138L vaccine.

Protein Peptide sequence Length (aa) Alleles Percentile ranka Antigenicity scoreb IFN-γ scorec Immunogenicity scored ABC pred scoree AllergenFP v.2.0f Toxin Predg IL-4h IL-10i
HTL epitopes
Ag85a VSGLVGAVGGTATAGA 16 HLA-DQA1*05:01/DQB1*03:01/
DQB1*06:02		 0.39 1.184 0.80257189 Non Non Non Non
GLVGAVGGTATAGAF 15 HLA-DQA1*05:01/DQB1*03:01 0.27 0.840 0.47981667 Non Non Non Non
SGLVGAVGGTATAGA 15 HLA-DQA1*05:01/DQB1*03:01 0.21 1.245 0.59277922 Non Non Non Non
VSGLVGAVGGTATAG 15 HLA-DQA1*05:01/DQB1*03:01
/DPB1*04:02/DRB1*01:01/DRB1*09:01		 0.31 1.203 0.49398202
Ag85b GLAGGAATAGAFSRPGL 17 HLA-DQA1*05:01/DQB1*03:01 0.37 0.542 1.5668744 Non Non Non Non
LPGLVGLAGGAATAGA 16 HLA-DQA1*05:01/DQB1*03:01 0.45 0.786 1.0183619 Non Non Non Non
VGLAGGAATAGA 12 HLA-DQA1*05:01/DQB1*03:01 0.27 1.282 0.52408286
AAIGLSMAGSSAMILAA 17 HLA-DRB1*09:01/DQA1*01:02
HLA-DQB1*06:02		 0.26 0.689 1.1091804
Mtb39A ARMYAGPGSASLVAA 15 HLA-DQA1*05:01/DQB1*03:01 0.35 0.564 0.015333722 Non Non Non Non
Rv1980 SDPAYNINISLPSYYPDQ 18 HLA-DRB3*02:02/DRB1*09:01
HLA-DRB1*14:01		 0.41 1.047 0.8399143 Non Non Non Non
Rv3873 GSDKALAAATPMVVWLQ 17 HLA-DQA1*06:01/DQB1*03:03 0.36 0.533 0.55646741 Non Non Non Non
GSDKALAAATPMVVW 15 HLA-DQA1*06:01/DQB1*03:03 0.22 0.744 0.13794349
CTL epitopes
Ag85a RVWVYCGNGK 10 HLA-A*11:01,HLA-A*30:01
HLA-A*68:01		 0.42 0.715 0.10358 Non Non
GLPVEYLQV 9 HLA-A*02:01, HLA-A*02:03, HLA-A*02:06, HLA-A*02:06 0.27 0.706 0.05492 Non Non
MGPTLIGLAM 10 HLA-B*35:01/ HLA-B*07:02 0.47 0.524 0.19966
Ag85b NAAGGHNAVF 10 HLA-B*35:01,HLA-B*53:01
HLA-A*26:01		 0.33 1.475 0.16235 Non Non
NTPAFEWYY 9 HLA-A*26:01,HLA-C*03:04,
HLA-B*15:01		 0.1 1.945 0.4288 Non Non
NAAGGHNAV 9 HLA-A*68:02 0.2 1.995 0.12765
Mtb39A ATATATATL 9 HLA-A*68:02/*32:01 0.47 1.001 0.1821 Non Non
LPPEINSARM 10 HLA-B*35:01,HLA-B*51:01 0.4 1.064 0.12798
Rv1980 GGTHPTTTYK 10 HLA-A*11:01,HLA-B*15:02,
HLA-C*07:02		 0.02 1.627 0.13929 Non Non
VSIAPNAGL 9 HLA-C*03:04, HLA-C*01:02 0.16 0.764 0.1185
Rv3873 DAQAVELTAR 10 HLA-A*33:03 0.36 1.389 0.18465 Non Non
SLPEIAANHI 9 HLA-C*01:02 0.4 0.608 0.31512 Non Non
GINTIPIAL 9 HLA-A*02:01 0.49 0.861 0.2913
B cellular epitopes
Ag85a AGGYKASDMWGPKEDP 16 0.84 Non Non
QDAYNAGGGHNGVFDF 16 0.73 Non Non
Ag85b LRAQDDYNGWDINTPA 16 0.85 Non Non
YCGNGTPNELGGANIP 16 0.79 Non Non
Rv1980 YELNITSATYQSAIPP 16 0.86 Non Non
RKPITYDTLWQADTDP 17 0.88 Non Non
Rv3873 PGASQSTTNPIFGMPS 16 0.85 Non Non
VNTLFEKLEPMASILD 0.8 Non Non

Abbreviations: HTL, helper T lymphocyte; CTL, cytotoxic T lymphocyte; IFN-γ, interferon-γ; IL, interleukin.

a

The percentile ranking of the selected epitopes; the inclusion criterion was a ranking score <0.5.

b

The antigenicity score; epitopes with antigenicity score >0.5 were selected.

c

IFN-γ score; the epitopes with the highest positive scores were selected.

d

The immunogenicity score; epitopes were selected in order of score.

e

The linear B-cell epitope prediction score; epitopes were selected in order of score.

f

The result of the sensitization or toxicity test, “Non” indicates that the epitope was non-toxic or non-sensitizing.

g

The result of the Toxin; “Non” indicates that the epitope was non-toxic.

h

Stimulated cytokine IL-4 secretion. HTL epitopes were selected that do not secrete cytokine IL-4.

i

Stimulated cytokine IL-10 secretion. HTL epitopes were selected that do not secrete cytokine IL-10.