Figure 3.

ESCs-Exo promoted the proliferation of HSFBs. (a,c) HSFBs were treated with 20 or 40 μg/ml exosomes for 24 h and then subjected to EdU and DAPI staining for the analysis of cell proliferation. Scale bar: 50μm. (b,d) Ki-67, actin, and DAPI staining for the analysis of cell proliferation. Scale bar: 100μm. (e,f) Cell proliferation was measured by measuring the mRNA expression of PCNA and MKI67, respectively, in HSFBs. The data are presented as the mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared with the control. ESCs pidermal stem cells,HSFBs human skin fibroblasts