Figure 6.

ESCs-Exo promoted the phosphorylation of PKN1 and the expression of cyclins. HSFBs were treated with 20 or 40 μg/ml exosomes for 72 h and the mRNA expression levels of CCND1 (a), CCNE1 (b), and CCNA2 (c) in HSFBs were analyzed via qRT-PCR. The 0 μg/ml exosome treatment was used as a control and the expression levels were normalized to those of the control. (d) Protein expression levels of phosphorylated (p)-PKN1, PKN1, cyclin D1, cyclin E1, and cyclin A2 were analyzed via western blotting. GAPDH was used as a loading control. The 0 μg/ml exosome treatment was used as a control and the expression levels were normalized to those of the control (e-h). n = 3 for each group, and the experiments were repeated three times to confirm the results. The data are presented as the mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 compared with the control. ESCs pidermal stem cells, HSFBs human skin fibroblasts