Table 2.
Metal composition of the challenged cells as determined by ICP-MSa.
| Metals present in the strain-specific MultiTox metal mix | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | condition Metal/cell | 103 Cr | 103 Co | 103 Ni | 103 Cu | 103 Zn | 103 As | 103 Cd | 103 Hg |
| CH34 | No addition | 4.8 ± 3.5 | 27 ± 10 | 20 ± 12 | 19 ± 10 | 83 ± 10 | bQ | bQ | bQ |
| CH34 | EDTA | bQ | 8 ± 4 | 5 ± 3 | 7 ± 3 | 50 ± 12 | bQ | bQ | bQ |
| CH34 | MetalMix | 75.6 ± 29.2 | 727 ± 278 | 1343 ± 338 | 1587 ± 543 | 3558 ± 2283 | 3746 ± 1 380 | 85 ± 53 | 27 ± 6 |
| Chal. Conc., (µM)b | 19 | 347 | 761 | 241 | 461 | 1503 | 15 | 0.37 | |
| AE104 | no addition | bQ | 27 ± 2 | 7 ± 3 | 10 ± 2 | 70 ± 16 | bQ | bQ | bQ |
| AE104 | EDTA | bQ | 18 ± 2 | 7 ± 3 | 11 ± 5 | 59 ± 12 | bQ | bQ | bQ |
| AE104 | MetalMix | 37.9 ± 8.7 | 61 ± 16 | 59 ± 18 | 2035 ± 2251 | 92 ± 9 | 2034 ± 1 933 | 236 ± 233 | 21 ± 11 |
| Chal. Conc., (µM)b | 7 | 7 | 9 | 120 | 5 | 849 | 3 | 0.18 | |
| Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM) | |||||||||
| Strain | condition metal | 106 Mg | 103 Ca | Mn | 103 Fe | Mo | |||
| CH34 | No addition | 10.7 ± 1.2 | 212 ± 92 | 531 ± 157 | 735 ± 80 | 2488 ± 359 | |||
| CH34 | EDTA | 9.6 ± 0.3 | 117 ± 23 | 680 ± 295 | 636 ± 60 | 2117 ± 100 | |||
| CH34 | MetalMix | 9.0 ± 0.5 | 213 ± 75 | 833 ± 311 | 609 ± 61 | 922 ± 41 | |||
| AE104 | No addition | 10.0 ± 0.9 | 164 ± 17 | 464 ± 175 | 740 ± 36 | 2670 ± 272 | |||
| AE104 | EDTA | 9.4 ± 0.4 | 154 ± 21 | 328 ± 134 | 675 ± 48 | 2378 ± 100 | |||
| AE104 | MetalMix | 10.0 ± 0.5 | 153 ± 41 | 491 ± 340 | 652 ± 55 | 2222 ± 48 | |||
a Cupriavidus metallidurans strains CH34 and AE104 were challenged with 50 µM EDTA, 3.35 mM of the CH34-specific, or 1 mM of the AE104-specific MultiTox metal mix. The metal content of the cells was determined by ICP-MS (n > 3). Bold faced letters indicate a significant (D > 1) up- and letters in italics a significant down-regulation compared to the unchallenged cells of the same strain; bQ, below limit of quantification (LOQ). bConcentration of an individual metal ion in the strain-specific metal mix. Please refer to the methods for details.