Table 2.
Products of the plasmid-encoded metal-resistance determinantsa
| Locus tag | Gene | CH34_0 | CH34_M | CH34_E | Description |
|---|---|---|---|---|---|
| Plasmid pMOL28: | |||||
| pMOL28 mer: not found merR, merT, merD, and merE | |||||
| Rmet_6346 | merP | NF | 3 935 ± 1757 | 48 | Periplasmic mercury-binding protein |
| Rmet_6183 | merA | NF | 11 249 ± 2 250 | NF | A6UXG5 Mercuric reductase |
| chr: not found chrZ, chrP, chrF1, chrA1, and chrI | |||||
| Rmet_6195 | chrY | NF | 263 ± 152 | NF | Q1L9 X 2 Putative uncharacterized protein |
| Rmet_6197 | chrN | NF | 129 ± 77 | NF | Q1L9 X 0 Putative uncharacterized protein |
| Rmet_6198 | chrO | NF | 727 ± 239 | 17 | Q1L9W9 Putative uncharacterized protein |
| Rmet_6200 | chrE | NF | 314 ± 199 | NF | Q5NUZ8 Superoxide dismutase SodM |
| Rmet_6201 | chrC | 104 | 1 318 ± 1 154 (12.7; 1.1) | NF | P17550 Superoxide dismutase (Fe) |
| Rmet_6203 | chrB1 | NF | 649 ± 516 | NF | P17552 Protein ChrB |
| cnr: not found cnrY, cnrC, and cnrA | |||||
| Rmet_6206 | cnrX | 44 | 388 ± 176 | NF | P37975 Nickel sensor of the antisigmafactor complex |
| Rmet_6207 | cnrH | 67 | NF | NF | P37978 RNA polymerase sigma factor CnrH |
| Rmet_6209 | cnrB | 207 | 1 235 ± 272 | NF | P37973 Nickel and cobalt-resistance protein CnrB |
| Rmet_6211 | cnrT | 11 | NF | NF | Q9L3G0 CnrT protein |
| Plasmid pMOL30: neither ncc nor sil products found | |||||
| pbr: not found pbrU, pbrR, pbrA, pbrB/C, and pbrD | |||||
| Rmet_5945 | pbrT | 206 ± 156 | 223 ± 47 (1.1; 0.1) | 847 ± 353 (4.1; 1.3) | Q58AJ4 PbrT protein (iron permease FTR1) |
| czc region: not found flgB, ompP, czcJ, czcD, czcI, czcN, and czcM | |||||
| Rmet_5970 | czcP | 31 ± 19 | 76 | NF | Q1LAJ7 Heavy metal translocating P-type ATPase |
| Rmet_5976 | czcE | NF | 96 | NF | Q1LAJ1 Putative uncharacterized protein |
| Rmet_5977 | czcS | NF | 313 ± 270 | NF | Q44007 Sensor protein CzcS |
| Rmet_5978 | czcR | 67 | 566 ± 539 (8.5; 0.9) | 89 ± 52 (1.3; 0.4) | Q44006 Transcriptional activator protein CzcR |
| Rmet_5980 | czcA | 95 | 854 ± 489 (9.0; 1.4) | 75 ± 50 (0.8; 0.2) | P13511 Cobalt–zinc–cadmium-resistance protein CzcA |
| Rmet_5981 | czcB | 146 ± 104 | 1 509 ± 607 (10.4; 1.9) | 113 ± 26 (0.8; 0.2) | P13510 Cobalt–zinc–cadmium-resistance protein CzcB |
| Rmet_5982 | czcC | 17 | 391 ± 207 (22.7; 1.8) | NF | P13509 Cobalt–zinc–cadmium-resistance protein CzcC |
| cop1: not found copV, copT, copM, copK, copD1, copJ, copG, copL, copQ, copE, and copW | |||||
| Rmet_6109 | copN | NF | 112 ± 25 | NF | Q1LA58 Putative uncharacterized protein |
| Rmet_6110 | copS1 | NF | 467 ± 471 | NF | Q58AD4 Sensor protein |
| Rmet_6111 | copR1 | 21 ± 9 | 322 ± 125 | 22 ± 8 (1.0; 0.1) | Q58AD5 Two-component regulator |
| Rmet_6112 | copA1 | NF | 1 054 ± 623 | NF | Q58AD6 Copper-resistance protein CopA |
| Rmet_6113 | copB1 | NF | 1 559 ± 870 | NF | Q58AD7 CopB protein (copper-resistance B) |
| Rmet_6114 | copC1 | NF | 704 ± 120 | NF | Q1LA53 Copper-resistance protein CopC |
| Rmet_6116 | copI | NF | 2 274 ± 982 | NF | Q58AE0 Putative oxydoreductase |
| Rmet_6119 | copF | 195 ± 116 | 768 ± 701 (3.9; 0.7) | 46 ± 21 (0.2; 1.1) | Q58AE3 Heavy metal translocating P-type ATPase |
| Rmet_6122 | copH | NF | 5 301 ± 2 837 | 36 | Q58AE5 CopH protein |
| pMOL30 mer1: no products found | |||||
| pMOL30 mer2: not found merT, merD, and merE | |||||
| Rmet_6171 | merR | 117 ± 68 | 151 ± 102 (1.3; 0.2) | 146 ± 38 (1.3; 0.3) | P69413 Mercuric-resistance operon regulatory protein |
| Rmet_6173 | merP | NF | 3 935 ± 1 757 | 48 | Q58AI1 Periplasmic mercuric ion-binding protein |
| Rmet_6174 | merA | NF | 249 ± 147 | NF | Q1L9Z3 Mercuric reductase MerA |
aThe copy numbers per cell of the products of plasmid-encoded metal-resistance determinants are given for C. metallidurans strain CH34 cultivated without added substance (CH34_0), metal-shocked (CH34_M) and metal-starvation conditions (CH34_E) with the mean values and deviations. Numbers without deviations indicate proteins determined just once in the respective triplicate determination. The copy numbers are followed by the ratios Q and the distance value D for the comparison of CH34_M and of CH34_E with CH34_0. These values were not provided when the respective protein could not be measured in CH34_0. NF, not found.