Table 4.
Metal uptake systemsa
| Locus tag | Gene | Control (Q, D) | Metal-shocked (Q, D) | Metal-starved (Q, D) | Description |
|---|---|---|---|---|---|
| Strain CH34 | |||||
| Rmet_3052 | corA1 | 394 ± 242 | NF | NF | Q1LIV2 Mg and Co transport protein CorA1 |
| Rmet_0036 | corA2 | 391 | NF | NF | Q1LSF4 Mg and Co transport protein CorA2 |
| Rmet_3287 | corA3 | 162 ± 96 | 180; (1.1; 0.2) | 108 ± 63 (0.7; 0.3) | Q1LI67 Mg and Co transport protein CorA3 |
| Rmet_1973 | pitA | NF | NF | NF | Q1LLX4 Phosphate transporter |
| Rmet_5396 | mgtA | 29 ± 17 | 32 (1.1; 0.2) | 26 ± 15 (0.9; 0.1) | Q1LC71 ATPase, E1–E2 type |
| Rmet_0549 | zntB | 82 ± 22 | 79 (1.0; 0.2) | 68 ± 19 (0.8; 0.3) | Q1LQZ1 Mg and Co transport protein ZntB |
| Rmet_5890 | feoB | 212 ± 69 | 292 ± 75 (1.4; 0.6) | 1176 ± 699 (5.6; 1.3) | Q1LAS7 Ferrous iron transport protein FeoB |
| Rmet_5891 | feoA | 194 | NF | 246 ± 142 (1.3; 0.4) | Q1LAS6 Ferrous iron transport protein FeoA |
| Strain AE104 | |||||
| Rmet_3052 | corA1 | 371 | NF | NF | Q1LIV2 Mg and Co transport protein CorA1 |
| Rmet_0036 | corA2 | 195 ± 128 (0.5; 1.5) | NF | 229 (1.2; 0.3) | Q1LSF4 Mg and Co transport protein CorA2 |
| Rmet_3287 | corA3 | 67 (0.4; 1.0) | 41 ± 26 (0.6; 1.0) | 127 ± 77 (1.9; 0.8) | Q1LI67 Mg and Co transport protein CorA3 |
| Rmet_1973 | pitA | 209 | NF | NF | Q1LLX4 Phosphate transporter |
| Rmet_5396 | mgtA | 22 (0.8; 0.4) | NF | 28 ± 17 (1.3; 0.4) | Q1LC71 ATPase, E1–E2 type |
| Rmet_0549 | zntB | 80 ± 52 (1.0; 0.0) | 186 (2.3; 2.0) | 51 ± 30 (0.6; 0.3) | Q1LQZ1 Mg and Co transport protein ZntB |
| Rmet_5890 | feoB | 311 ± 118 (1.5; 0.5) | 443 ± 432 (1.4; 0.2) | 588 ± 216 (1.9; 0.8) | Q1LAS7 Ferrous iron transport protein FeoB |
| Rmet_5891 | feoA | NF | 178 | 125 ± 72 | Q1LAS6 Ferrous iron transport protein FeoA |
aThe number of the products per cell of the respective gene with deviations, plus the comparisons CH34 with metal mix to without, and CH34 with EDTA to without, same for strain AE104 and for nonchallenged control cells AE104/CH34. The ratios Q and the distance values follow these numbers in parentheses. Single values indicate a result only in one out of the three determinations. NF is ‘not found’ in any of the three replicates. Comparisons to NF values were not done. Bold-faced Q ratios with D >1 and at least two-fold up- und downregulation of the abundance.