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. 2024 Nov 5;300(12):107972. doi: 10.1016/j.jbc.2024.107972

Figure 1.

Figure 1

HSA and HODE derived FAHFAs are upregulated in adipose tissue upon LPS treatment in vivo. RAW cells were treated with 100 μM (A) 12-HSA, (B) 9-HODE, or (C) 13-HODE along with 20 mM C17:1 for 2 h. HSA and HODE-derived FAHFAs were measured via targeted LC-MS (n = 2 per group). D, experimental scheme for (E–G). Mice were injected with 3 mg/kg LPS i.p. Tissues were collected at t = 0 h, 8 h, and 24 h. FAHFAs in (E) PGWAT, (F) SQWAT, and (G) BAT were measured via targeted LC-MS (n = 3–4 per group. ∗, p < 0.05; ∗∗, p < 0.01; and ∗∗∗, p < 0.001, compared with t = 0. t test). Error bars represent SD. FAHFA, fatty acid hydroxy fatty acid; HETE, hydroxyeicosatetraenoic acid; HODE, hydroxyoctadecadienoic; i.p., intraperitoneally; LPS, lipopolysaccharide.