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. 2024 Nov 5;300(12):107972. doi: 10.1016/j.jbc.2024.107972

Figure 2.

Figure 2

13-LAHODE has anti-inflammatory activity in vivo. RAW cells were treated with vehicle (control), or 100 ng/ml LPS with or without 5 μM dexamethasone or indicated concentrations of 13-LAHODE for 24 h. A, IL-6 levels were measured via ELISA. B, cell viability was inferred using MTT assay (n = 3 per group. ∗, p < 0.05; ∗∗, p < 0.01; and ∗∗∗, p < 0.001, compared with LPS. t test). C, experimental scheme for in vivo anti-inflammatory assay. Mice were injected with 10 mg/kg 13-LAHODE or vehicle at t = −1 h. Then, injected with 0.15 mg/kg LPS. Plasma samples were collected at t = 0, 1 h, 2 h, and 6 h. Plasma (D) TNF and (E) IL-6 levels were measured by ELISA (n = 5 per group. ∗, p < 0.05, compared with vehicle. t test). Error bars represent SD. 13-LAHODE, linoleic acid ester of 13-HODE.