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. 2024 Nov 5;300(12):107972. doi: 10.1016/j.jbc.2024.107972

Figure 5.

Figure 5

Other oxylipins contribute to synthesis of FAHFAs with anti-inflammatory activity in vitro. RAW cells were treated with 100 μM (A) 9-HETE, (B) 15-HETE, or (C) LTB4 along with 20 mM C17:1 for 2 h. HSA and HODE-derived FAHFAs were measured via targeted LC-MS (n = 2 per group). RAW cells were treated with vehicle (control), or 100 ng/ml LPS with or without 5 μM dexamethasone or indicated concentrations of 5-SALTB4 or 12-SALTB4 for 24 h. D, IL-6 levels were measured via ELISA. E, cell viability was inferred using MTT assay (n = 3 per group. ∗p < 0.05; ∗∗p < 0.01; and ∗∗∗p < 0.001, compared with LPS. t test). Error bars represent SD. HODE, hydroxyoctadecadienoic; HETE, hydroxyeicosatetraenoic acid; FAHFA, fatty acid hydroxy fatty acid; LTB4, leukotriene B4; SALTB4, stearic acid LTB4.