Figure 3.

CAR activation induces proinflammatory gene programs in Tregs

(A) Heatmap clustered by column (sample) and by row (gene) with top 100 most differentially expressed genes between No Act Tregs, TCR Tregs, CAR Tregs, No Act Teff, TCR Teff, and CAR Teff. (B) Venn diagram with genes upregulated in TCR Tregs, CAR Tregs, TCR Teff, and CAR Teff in relation to their respective No Act cell types. Number of genes and respective percentage of the total number of genes are indicated in each intersection. (C) Top 20 protein-coding genes most differentially expressed in CAR Tregs compared with TCR Tregs. FC, fold change; padj, adjusted p value. (D) KEGG pathway gene set enrichment analysis (GSEA) of CAR Tregs vs. TCR Tregs. FDR, false discovery rate.