Figure 5.

CAR activation induces CD40L expression in Tregs

(A) CD40L and TIGIT surface expression on No Act Tregs, TCR Tregs, and CAR Tregs 48 h post-activation. Representative histograms on the left and summary data on the right. (B) CD40L and TIGIT surface expression on No Act Tregs, TCR Tregs, and CAR Tregs 1 week post-activation. Representative histograms on the left and summary data on the right. (C) Expression of selected genes in CAR Tregs and TCR Tregs 24 h post-activation, evaluated by qPCR. Bars represent mean ± standard deviation (SD) technical replicates of representative experiment. Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparison correction (A and B) and unpaired Student’s t test (C). ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05; ns, not significant.