Figure 8.

CD40L expression is associated with IFN-γ production in CAR Tregs

(A) CD40L surface expression in TCR Tregs, CAR Tregs, and CAT Tregs 18 h post-activation. Representative histograms on the left and summary data on the right. (B) TIGIT surface expression in TCR Tregs, CAR Tregs, and CAT Tregs 18 h post-activation. Representative histograms on the left and summary data on the right. (C) Relative frequency of TIGIT⁻CD40L^low, TIGIT⁺CD40L^low, TIGIT⁻CD40L^hi, and TIGIT⁺CD40L^hi cells among TCR Tregs, CAR Tregs, and CAT Tregs 18 h post-activation. (D) Frequency of IFN-γ-producing cells among TIGIT⁻CD40L^low, TIGIT⁺CD40L^low, TIGIT⁻CD40L^hi, and TIGIT⁺CD40L^hi subpopulations for TCR Tregs, CAR Tregs, and CAT Tregs 18 h post-activation. For (A), (B), and (D), values represent technical replicates of representative experiment. Bars represent mean ± standard deviation (SD). Statistical significance was assessed using one-way ANOVA test with Tukey’s multiple comparison correction. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05; ns, not significant.