Figure 7.

The TPR domain of SPY interacts with the SPY protein in a yeast two-hybrid assay. The β-galactosidase activity of yeast strains containing plasmids expressing a GAL4 DNA binding domain: TPR fusion protein (TPR-DB), GAL4 DNA binding domain: SPY fusion protein (SPY-DB), the GAL4 DNA binding domain (DB), GAL4 activation domain:SPY fusion protein (SPY-AD), or the activation domain (AD) was determined. The proteins expressed in each strain are indicated below. In some cases, the se is too small for the se bar to be visible. The experiment was repeated two additional times with similar results.