Subject | Biological Sciences |
Specific subject area | Omics: Genomics |
Type of data | Table: Gene annotations, base composition Figures: Zhangixalus omeimontis, circular mitogenome map, phylogenetic tree Fasta: Mitogenome sequence Fastq: DNA sequence reads Data Format: Raw and analyzed |
Data collection | DNA Extraction and Sequencing: Genomic DNA was extracted from the frog toe pad using the TIANamp Animal Genomic DNA Kit (Tiangen Biotech, Beijing). Assembly and annotation: PCR amplification of the 15 pairs of gene primers designed was performed. The PCR amplification fragments were separated by 0.9 % agarose gel electrophoresis and imaged using a gel imager. The purified amplification products were subjected to automated direct sequencing using the Sanger sequencing method and an ABI 3730 sequencer. The sequencing products were assembled into complete mitochondrial genome sequences using DNA Baser software (http://www.DNABaser.com) based on the overlapping regions (150–300 bp overlapping regions) and the annotation process was performed using MitoMaker. Phylogenetic analysis: IQ-tree was used to construct the Maximum Likelihood phylogenetic tree. |
Data source location | Location: Baicha Village, Bailu Town City: Pengzhou City, Sichuan Province Country: China Latitude and Longitude: 31°13′18.07″N, 103°54′10.28″E Sample Storage Facility: Genomic DNA was deposited in the Ecological Security and Protection Key Laboratory of Sichuan Province with the voucher number JL20200812 collection overseen by Lichun Jiang; contact email: jiang_lichun@126.com). |
Data accessibility | Repository name: GenBank Data identification number: MZ936366 Direct URL to data: https://www.ncbi.nlm.nih.gov/nuccore/MZ936366/ Repository name: NCBI BioProject Data identification number: PRJNA1001901 Direct URL to data: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1001901 Repository name: NCBI BioSample Data identification number: SAMN36836140 Direct URL to data: https://www.ncbi.nlm.nih.gov/biosample/?term= SAMN36836140 Repository name: NCBI SRA Data identification number: SRR25517597 Direct URL to data: https://www.ncbi.nlm.nih.gov/sra/?term= SRR25517597 Repository name: NCBI Genbank Data identification number: MZ936366 Direct URL to data: https://www.ncbi.nlm.nih.gov/nuccore/ MZ936366 Repository name: Zenodo data Data identification number: 10.5281/zenodo.13898565 Direct URL to data: https://zenodo.org/records/13898566 |
Related research article |