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. 2024 Dec 16;96(12):e70118. doi: 10.1002/jmv.70118

Figure 2.

Figure 2

IFI16 co‐elutes with DDX5 in high MW protein complexes containing PRC2. (A) Workflow of nuclear protein complex identification by size exclusion chromatography (SEC). Nuclear lysates prepared under non‐denaturing conditions from FLAG‐DDX5 expressing HepaRG cells, fractionated by Superdex 200 10/300 GL column and AKTA fast protein LC system. (B) MW (kDa) of complexes eluting in indicated SEC fractions. (C) Immunoblots of SEC fractions (#15–22) for indicated proteins. (D) Label‐free quantification (LFQ) of indicated proteins identified by LC/MS/MS in fractions #17–20 (n = 2). (E) DDX5 immunoprecipitates, from HepaRG native nuclear lysates, analyzed by native polyacrylamide gel electrophoresis. Immunoblots of DDX5, IFI16, SUZ12 and RBBP4 are shown. Non‐specific binding of antibodies to unstained MW marker, visualized by coomassie blue staining of PVDF membrane. A representative assay is shown from n = 3.