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. 2024 Dec 2;102:skae365. doi: 10.1093/jas/skae365

Figure 3.

Figure 3.

The relative gene expression of markers of satellite cell development was analyzed in non-steroidal implanted (NoIMP) and steroidal implanted (TE200) steers that were supplemented 0, 100, or 150 mg Zn/kg DM (Zn0, Zn100, Zn150, respectively). Samples from the longissimus thoracis were collected on day 11 and relative gene expression was calculated relative to NoIMP-Zn0 treatment. Contrast statements were formed to test the effect of increasing Zn from Zn0 to Zn150 within NoIMP (Zn-NoIMP) and TE200 (Zn-TE200), in addition to the main effect of steroidal implant (IMP; Zn0 and Zn150) and the linear effect of increasing Zn as Zn0, Zn100, and Zn150 within TE200 (Zn-L). A) Myogenic differentiation 1 (MYOD) gene expression was not influenced by the tested contrast statements (P ≥ 0.26). B) Myogenin (MYOG) and C) myogenic factor 5 (MYF5) gene expression were greater for TE200 than NoIMP for Zn0 and Zn150 treatments (P ≤ 0.04). D) Paired box protein 7 (PAX7) gene expression was not influenced by the tested contrast statements (P ≥ 0.11).