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. 2024 Dec 2;102:skae365. doi: 10.1093/jas/skae365

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© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Relative gene expression of Zn transporters in the muscle of non-steroidal implanted (NoIMP) and steroidal implanted (TE200) steers supplemented 0, 100, or 150 mg Zn/kg DM (Zn0, Zn100, Zn150, respectively). Samples were collected on day 11 from the longissimus thoracis and relative gene expression was determined relative to NoIMP-Zn0 treatment. Contrast statements were formed to test the effect of increasing Zn from Zn0 to Zn150 within NoIMP (Zn-NoIMP) and TE200 (Zn-TE200), in addition to the main effect of steroidal implant (IMP; Zn0 and Zn150) and the linear effect of increasing Zn as Zn0, Zn100, and Zn150 within TE200 (Zn-L). A) Solute carrier family 30 member 7 (SLC30A7) gene expression tended to be greater for Zn150 than Zn0 within TE200 steers (P = 0.07) and tended to linearly increase with increasing Zn supplementation (Zn0, Zn100, and Zn150) within TE200 (P = 0.06). Gene expression of SLC30A7 was lesser for TE200 than NoIMP (P = 0.05). B) Solute carrier family 39 member 7 (SLC39A7) and C) solute carrier family 39 member 14 (SLC39A14) were not affected by the tested contrast statements (P ≥ 0.11).