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. 2024 Dec 5;121(50):e2411402121. doi: 10.1073/pnas.2411402121

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Copyright © 2024 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 4. — Importance of the actin cytoskeletal network in molecular size-dependent diffusion suppression. (A) 3D-STORM superresolution images of phalloidin-labeled actin filaments in actin-preserved (Top) and actin-supplemented (Bottom) Xenopus egg extracts. Color presents axial (depth) information. (B) SMdM-determined D values in the extract relative to in PBS, for the ~30 kDa BCA (black) and the ~660 kDa thyroglobulin (red), in actin-inhibited, actin-intact, and actin-supplemented samples. Each data point corresponds to one independent SMdM measurement for a different sample region, from ~3 samples under each condition.