Fig. 1.

A) irCLIP workflow adapted from Zarnegar et al. 2016 (Zarnegar et al. 2016). B) irCLIP in nuclear and cytosolic fraction of HeLa. Anti-hnRNPA2B1 and anti-Galectin-3 identify RNA primarily in the nuclear fraction. C) irCLIP-MS in-gel digest and proteomic identification of proteins associated with irCLIP signal. Galectin-3 is not detected in the region of the gel containing RNA signal. D) Fractionation of HeLa cells. Demonstration of separation of nuclei (Lamin A/C) from cytosol (Tubulin). Anti-Galectin-3 mAb [EPR19244] detects bands in both the cytoplasmic and nuclear fractions. E) Enrichment profiles of two anti-galectin-3 antibodies by IP-MS. Both antibodies enrich well characterized RNA-binding proteins.