Table 1:

Nuclei isolation lysis buffer and wash buffer composition.

Lysis Buffer
Reagent Name	Stock Concentration	Final Concentration	Total Volume 1 mL
Tris-HCL (Ph7)	1 M	10 mM	10 μL
NaCl	5 M	10 mM	2 μL
MgCl2	0.1 M	3 mM	3 μL
Tween-20	20%	0.10%	5 μL
Nondiedt P40	10%	0.10%	10 μL
Digitonin	5%	0.01%	2 μL
DTT	1 M	1 mM	1 μL
RNase inhibitor	40 U/μL	1 U/μL	25 μL
Nuclease-free Water	--	--	924 μL
Wash Buffer
Reagent Name	Stock Concentration	Final Concentration	Total Volume 1 mL
BSA in PBS	10%	1%	100 μL
PBS	--	--	900 μL