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. 2024 Dec 13;12:RP90419. doi: 10.7554/eLife.90419

Figure 6. Loss of Slc39a5 improves hepatic inflammation and fibrosis in female mice challenged with diet-induced non-alcoholic steatohepatitis (NASH).

Slc39a5-/- and Slc39a5+/+ mice were placed on a NASH-inducing diet or NC for 40 wk and sacrificed after 16 hr of fasting. (A, B) NASH Slc39a5-/- mice display reduced serum ALT and AST levels. (C–E) Histology scores for steatosis, hepatocyte hypertrophy, and inflammation. (F) NAFLD activity score was reduced in NASH Slc39a5-/- mice. (G–I) NASH Slc39a5-/- mice display reduced fibrosis. (G) Representative images of explanted livers sample stained with picrosirius red indicative of collagen deposition. Scale bar, 300 µm. (H, I) Fibrosis and steatosis-activity-fibrosis scores. n=6–7 (NC) and 8–11 (NASH), *p<0.05, **p<0.01, ***p<0.001, two-way ANOVA with post hoc Tukey’s test. Numeric data is summarized in Supplementary file 6.

Figure 6.

Figure 6—figure supplement 1. Loss of Slc39a5 reduces hepatic inflammation and fibrosis in male mice challenged with diet-induced non-alcoholic steatohepatitis (NASH).

Figure 6—figure supplement 1.

Mice were fed NASH diet or NC for 40 wk and sacrificed after 16 hr fasting. (A–B) Loss of Slc39a5 reduces serum ALT and AST levels (biomarkers of liver damage). (C–E) Histology scores for steatosis, hepatocyte hypertrophy, inflammation. (F) Non-alcoholic fatty liver disease (NAFLD) activity score. (G–I) Loss of Slc39a5 improves fibrosis in mice upon NASH dietary challenge. (G) Representative images of explanted livers sample stained with picrosirius red indicative of collagen deposition. Scale bar, 300 µm. (H–I) Histology score for fibrosis and steatosis-activity-fibrosis score. n=7–10 (NC) and 15–17 (NASH), *p<0.05, **p<0.01, ***p<0.001, two-way ANOVA with post hoc Tukey’s test. Numeric data is summarized in Supplementary file 6.
Figure 6—figure supplement 2. Loss of Slc39a5 improves liver function in mice challenged with diet-induced non-alcoholic steatohepatitis (NASH).

Figure 6—figure supplement 2.

Female (A–E) and Male (F–J) mice. (A, F) Body weight. (B, G) Fasting blood glucose. (C, H) Serum zinc. (D, I) Hepatic zinc. (E, J) Total hepatic superoxide dismutase (SOD) activity. n=6–10 (NC) and 8–17 (NASH), *p<0.05, **p<0.01, ***p<0.001, two-way ANOVA with post hoc Tukey’s test.