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. Author manuscript; available in PMC: 2024 Dec 16.
Published in final edited form as: Nat Biotechnol. 2022 Apr 7;40(8):1285–1294. doi: 10.1038/s41587-022-01240-2

Extended Data Fig. 6 |. Fludarabine does not increase transduction of CRISPR/Cas9 encoded rAAV vectors or saCas9 mRNA.

Extended Data Fig. 6 |

a-b, At the conclusion of the two-week experiment in Fig. 5, DNA was extracted from livers of rAAV-injected animals. Viral genomes were quantified by qPCR and normalized to cellular genomic DNA. The donor rAAV repair template AAV-GFP-HDR is presented on the top panel (a), while AAV-Cas9 is displayed in the middle panel (b). Each point represents data from one animal, with n = 3 to 5 mice per group. Error bars represent s.d. Significance testing was performed using two-tailed t tests or Mann-Whitney U test if data was non-normally distributed. c, Mice were injected with SaCas9 (6.0E12 vg/kg) following fludarabine or PBS treatment as in Fig. 5f–g. Two weeks later, total RNA was extracted and saCas9 mRNA levels quantified by qPCR. n = 3 mice per group. Error bars represent s.d. Significance was determined using two-tailed t test.