Skip to main content
. Author manuscript; available in PMC: 2024 Dec 16.
Published in final edited form as: Nat Biotechnol. 2022 Apr 7;40(8):1285–1294. doi: 10.1038/s41587-022-01240-2

Extended Data Fig. 2 |. RNAScope in situ hybridization of mAlb and hF9 in mouse liver.

Extended Data Fig. 2 |

a, Detection of hF9 mRNA (red) in liver sections using RNAScope in situ hybridization. Liver sections of mice from non-injected, PBS-treated, and Flu-treated groups at the end of experiment in Fig. 2 (60 days after rAAV transduction, ~12 weeks of age) were used for hybridization and counterstained with hematoxylin. Representative images from each injected group are shown. b. Representative images of RNAScope in situ hybridization are provided. In the first two columns, mAlb mRNA was stained in a non-injected normal mouse to determine the albumin locus expression characteristics. Specificity for RNA was confirmed by digestion of tissue with RNAseA. c, Additional images of hF9 mRNA staining in mice injected with Alb-P2A-hF9 vector, with or without fludarabine treatment are shown at various levels of magnification.