Table 3.
Ca2+ transient parameters for loaded CTRL and HFSD mouse cardiomyocytes
| CTRL | HFSD | P | |||||
|---|---|---|---|---|---|---|---|
| 2 Hz | 4 Hz | 2 Hz | 4 Hz | Frequency | Diet | Interaction | |
| Systolic Ca2+ (F 340:380) | 0.93 ± 0.08 | 1.05 ± 0.10# | 0.99 ± 0.15 | 1.08 ± 0.20 | 0.0004 | 0.571 | 0.100 |
| Amplitude Ca2+ (F 340:380) | 0.26 ± 0.06 | 0.28 ± 0.06 | 0.32 ± 0.10 | 0.33 ± 0.11 | 0.569 | 0.0672 | 0.723 |
| Time constant of Ca2+ decay (τ, s) | 0.21 ± 0.07 | 0.09 ± 0.02# | 0.17 ± 0.07 | 0.09 ± 0.03# | <0.0001 | 0.207 | 0.350 |
Comparisons in data with two independent variable were performed using a mixed analysis 2‐way ANOVA with Šidák's post hoc analysis (CTRL, 2 Hz: N = 5 mice, n = 13 cells; CTRL, 4 Hz: N = 4 mice, n = 9 cells; HFSD, 2 Hz: N = 5 mice, n = 14 cells; HFSD, 4 Hz: N = 4 mice, n = 8 cells). Data are presented as means ± standard deviation. P‐values for ANOVA effects (Frequency, Diet, Interaction) are listed within the table. #Significant pairwise post hoc frequency differences vs. CTRL 2 Hz or HFSD 2 Hz, P < 0.05. No pairwise post hoc treatment differences were observed.