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Expression of the Treg cell lineage transcription factors FOXP3 and HELIOS in CD4+ T cells activated via anti-CD3/CD28 dynabeads, untransduced (UT) Treg cells activated via anti-CD3/CD28 dynabeads, or CD19CAR Treg cells activated with irradiated CD19-K562 cells for 9 days. Dot plots represent FOXP3 and HELIOS expression in viable CD4+ cells, as detected by flow cytometry with anti-human FOXP3 eFluor 450 and anti-human HELIOS PE.
