Figure 3.

Heatmap of antibiotic resistance genes (ARGs) with > 1% relative quantification in all pooled samples or found in both HT-qPCR and WMGS. Data scaled by the minimal non-zero value across all analyzed samples. The upper left part of each cell corresponds to the workplace (Work) samples, and the lower right part to the household (Home) samples, facilitating easy paired comparison. Circles inside cells indicate orders of magnitude for better readability. Cross marks (✘) denote ARGs that were found also by WMGS. ENT, pediatric hospital; NEO, maternity hospital; and RCX, research center; MDR, multidrug resistance; MLSB, genes associated with the phenotype resistant to macrolides, lincosamides, and streptogramin B; HT-qPCR, high-throughput quantitative PCR; WMGS, whole metagenome shotgun sequencing.