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Kinetic parameters for the cathepsin B (CTSB)-catalyzed hydrolysis of Z-Arg-Arg-AMC and the cathepsin L (CTSL)-catalyzed hydrolysis of Z-Phe-Arg-AMC. All experiments were performed with 10 ng CTSB or CTSL in 100 mM sodium acetate buffer with 5 mM calcium chloride at pH 5.5 supplemented with 10 mM DTT
| KM (mM) | kcat (s−1) | kcat/KM (mM−1 s−1) | |
|---|---|---|---|
| CTSB | 0.37 ± 0.05 | 77.00 ± 28.90 | 208.11 ± 33.12 |
| CTSL | 0.02 ± 0.00 | 230.00 ± 43.50 | 9430.09 ± 337.42 |
