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. 2024 Nov 8;43(24):6573–6590. doi: 10.1038/s44318-024-00297-w

Figure EV2. TRMT10C/SDR5C1 interactions with ELAC2 and PRORP (related to Fig. 2).

Figure EV2

(A) ELAC2 cleavage of the mt-tRNAHis-GAG precursor in the absence of TRMT10C/SDR5C1, or with the mutations V256E_L257E that affect residues interacting with TRMT10C. (B) TRMT10C NTD interacts with ELAC2 (RNase Z, left panel) and PRORP (RNase P, right panel) in the T loop region. (C) The ELAC2 C-terminal helix would collide with a 5′ extension on the mt-tRNA precursor (yellow circle). To make this figure, the mt-tRNA precursor in RNase Z was replaced with the RNase P one (mt-tRNATyr), which has a 2-nucleotide 5′-extension. For this, The RNase Z structure was superimposed on the RNase P structure (Bhatta et al, 2021; PDB 7ONU), using TRMT10C as a reference. Source data are available online for this figure.